pNPP Phosphatase Assay Kit 500
PNPP Phosphatase Assay Kit:
P-Nitrophenylphosphate (pNPP) is a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The reaction produces para-nitrophenol, which becomes a deep yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer.
This homogeneous colorimetric “mix and measure” pNPP Phosphatase assay involves simply adding a single reagent to the phosphatase and measuring product formation in an absorbance reader. The assay can be conveniently performed in cuvettes, tubes, or multi-well plates at room temperature or 37 ° C.
Applications
For the quantitative determination of phosphatase activity.
PNPP Phosphatase Assay Kit – Key Features
- High sensitivity and wide linear range. The limit of detection is generally 3 ng of phosphatase or less.
- Simple and homogeneous procedure. No reagent wash or transfer steps are required. The test can be completed in 30 minutes.
- Robust and compatible with HTS. All reagents are compatible with high-performance liquid handling instruments.
PNPP Phosphatase Assay Kit Information:
Kit includes
Size (assays) 500, reagent 280 ml, assay buffer 25 ml, stop solution 25 ml. pNPP-HTS> 10k custom.
Detection method: OD405nm
Detection limit: 3 ng
Samples: Protein phosphatases
Species: all
Protocol duration: 30 min
Size: 500 tests
Storage: Store the reagent at -20 ° C and other components at 4 ° C.
Shelf life: 12 months.
