pNPP Phosphatase Assay Kit 500

PNPP Phosphatase Assay Kit:

P-Nitrophenylphosphate (pNPP) is a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The reaction produces para-nitrophenol, which becomes a deep yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer.

This homogeneous colorimetric “mix and measure” pNPP Phosphatase assay involves simply adding a single reagent to the phosphatase and measuring product formation in an absorbance reader. The assay can be conveniently performed in cuvettes, tubes, or multi-well plates at room temperature or 37 ° C.


For the quantitative determination of phosphatase activity.

PNPP Phosphatase Assay Kit – Key Features

  • High sensitivity and wide linear range. The limit of detection is generally 3 ng of phosphatase or less.
  • Simple and homogeneous procedure. No reagent wash or transfer steps are required. The test can be completed in 30 minutes.
  • Robust and compatible with HTS. All reagents are compatible with high-performance liquid handling instruments.

PNPP Phosphatase Assay Kit Information:

Kit includes

Size (assays) 500, reagent 280 ml, assay buffer 25 ml, stop solution 25 ml. pNPP-HTS> 10k custom.

Detection method: OD405nm

Detection limit: 3 ng

Samples: Protein phosphatases

Species: all

Protocol duration: 30 min

Size: 500 tests

Storage: Store the reagent at -20 ° C and other components at 4 ° C.

Shelf life: 12 months.

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