QuantiChrom™ Urea Assay Kit

Request

For the quantitative enzymatic determination of urea.

Key features

  • Fast and responsive. Linear detection range (20 μL sample): 1 mg / mL (0.17 mM) to 100 mg / mL (17 mM) of urea in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent and reading the absorbance after 5 minutes. Test at room temperature. No heater needed at 37 ° C.
  • High performance. Homogeneous “mix-incubate-measure” assay. It can be easily automated as a high throughput 96-well plate assay for thousands of samples per day.

Method: OD557nm

Samples

Urea in biological samples (eg plasma, serum, urine) and food/beverage samples (eg milk).

Species: All

Size: 100 tests

Detection limit: 1 mg / ml (0.17 mM)

Duration: 6 months

More details

UREA, the main end product of protein catabolism in animals, is produced primarily in the liver and is secreted by the kidneys. It is the primary vehicle for removing toxic ammonia from the body. The determination of urea is very useful for clinicians to evaluate the kidney function of patients. In general, elevated urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (eg, congestive heart failure, liver disease, and diabetes).

Decreased levels often indicate acute liver failure, but can also be the result of overly vigorous parenteral fluid therapy. The BioAssay Systems Colorimetric Urea Assay (BUN) is based on the urea catalyzed conversion of urea to ammonium and carbon dioxide. The pH of the reaction medium is controlled by a chromogen and the intensity of the reaction product at 557 nm is directly proportional to the concentration of urea in the sample.

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