Upregulated functional gene expression programmes

• Seth
• June 21, 2021May 25, 2021
• 0

Upregulated purposeful gene expression programmes in tumour pericytes mark development in sufferers with low-grade glioma

Pericytes conceivably play vital roles within the tumour microenvironment of glioblastoma multiforme (GBM) by permitting for an aberrant vasculature and performing as a element within the perivascular area of interest that helps glioma stem-like cells. Nevertheless, a scarcity of particular markers has hampered in-depth elucidation of the purposeful contribution of pericytes to GBM. This examine offers a complete computational biology strategy to annotate pericyte marker genes within the GBM vasculature by integration of knowledge from single-cell RNA-sequencing research of each mouse and human tissue, in addition to bulk tumour- and healthy-tissue gene expression information from sufferers with GBM.

We recognized distinct vascular- and immune-related gene expression programmes in tumour pericytes that we assessed for affiliation with GBM traits and affected person survival. Most compellingly, pericyte gene signatures that had been upregulated in tumours in comparison with regular mind tissue had been indicative of development of low-grade gliomas into high-grade glioma, recommended by a markedly shorter total survival. Our outcomes underline the purposeful significance of tumour pericytes in low-grade glioma and will function a place to begin for efforts for precision concentrating on of pericytes.

Mouse Monoclonal anti-Human CCL-11
hAP-0136A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-28
hAP-0153	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-28
hAP-0153A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-Human CCL-1
hAP-0135	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-22 (MDC)
hAP-0147	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-17 (TARC)
hAP-0141	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-17 (TARC)
hAP-0141A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-18 (PARC)
hAP-0142	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-25 (TECK)
hAP-0150	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-27 (CTACK)
hAP-0152	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-27 (CTACK)
hAP-0152A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-21 (6Ckine)
hAP-0146	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-24 (Eotaxin)
hAP-0149	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-5 (RANTES)
hAP-0155	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-5 (RANTES)
hAP-0155A	Angio Proteomie	100ug	EUR 250

Anti-Mouse CCL-11 Antibody
103-M321	ReliaTech	100 µg	EUR 399
Description: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Mouse CCL11 cDNA encodes a 97 amino acid (aa) precursor protein from which the aminoterminal 23 aa are cleaved to generate the 74 aa mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high aa sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFNγ. In addition, CCL11 mRNA is also induced in response to the transplantation of IL4secreting tumor cells. The CC chemokine receptor 3 (CCR3) has been identified as a specific human CCL11 receptor.

Anti-Mouse CCL-12 Antibody
103-M322	ReliaTech	100 µg	EUR 399
Description: CCL12 is a cloned mouse CC chemokine most closely related to human MCP1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

Anti-Mouse CCL-17 Antibody
103-M323	ReliaTech	100 µg	EUR 399
Description: Human thymus and activationregulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1α, MIP1β, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

Anti-Mouse CCL-19 Antibody
103-M324	ReliaTech	100 µg	EUR 399
Description: CCL19/MIP3β, also known as ELC (EBI1Ligand Chemokine), is a β chemokine that binds specifically to the chemokine receptor CCR7/EBI1/ BLR2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other β chemokines (20-30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is downregulated by the antiinflammatory cytokine IL10. Recombinant CCL19 has been shown to be chemotactic for Tcells and Bcells. The CCL19 receptor (CCR7/ EBI1/ BLR2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly upregulated in Bcells infected with EpsteinBarr virus and T-cells infected with herpesvirus 6 or 7.

Anti-Mouse CCL-20 Antibody
103-M326	ReliaTech	100 µg	EUR 399
Description: Macrophage inflammatory protein 3 alpha (MIP-3α) is a member of the CC chemokine superfamily and has been designated CCL20. CCL20 is a β chemokine that is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. CCL20 binds to CCR6 to promote chemotaxis of lymphocytes and inhibit the proliferation of myeloid progenitors.

Anti-Mouse CCL-21 Antibody
103-M327	ReliaTech	100 µg	EUR 399
Description: 6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoidtissue chemokine), CCL21 and Exodus2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxy-lterminal domain. Human 6Ckine cDNA encodes a 134 amino acid highly basic precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine share 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dosedependent manner. 6Ckine acts via the CC receptor CCR7 on T cells and B cells.

Anti-Mouse CCL-22 Antibody
103-M328	ReliaTech	100 µg	EUR 399
Description: CCL22, also known as ABCD1 and MDC (macrophage-derived chemokine), is a CC chemokine cloned from activated mouse B cells. Mouse CCL22 cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human CCL22 share 64% identity and 83% similarity. The genomic organization of the mouse and human CCL22 genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse CCL22 mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. CCL22 is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

Anti-Mouse CCL-24 Antibody
103-M329	ReliaTech	100 µg	EUR 399
Description: Eotaxin2, also named myeloid progenitor inhibitory factor (MPIF2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL4. LPS and IL4 also differentially regulate the expression of Eotaxin2 in monocytes and macrophages. Mouse Eotaxin2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin2. Functionally, Eotaxin2is most closely related to CCL11/Eotaxin and CCL26/Eotaxin3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

Anti-Mouse CCL-25 Antibody
103-M330	ReliaTech	100 µg	EUR 399
Description: CCL25 (thymus expressed chemokine), also known as TECK (thymus-expressed chemokine), is a CC chemokine that is distantly related (twenty some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8.

Anti-Mouse CCL-27 Antibody
103-M331	ReliaTech	100 µg	EUR 399
Description: CCL27, also known as CTACK (cutaneous T cel-lattracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively. It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL1, and determinants on the surface of fibroblasts and endothelial cells. CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation. Endothelial cellbound CCL27 can mediate the adhesion of those cells to CLA+ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

Anti-Mouse CCL-28 Antibody
103-M332	ReliaTech	100 µg	EUR 399
Description: Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) precursor protein with a 22 aa signal peptide and a 108 aa mature protein. Mature human and mouse CCL28 share 83% aa identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10, which is also the receptor for CCL27/CTACK.

Mouse Monoclonal anti-human CCL-13 (MCP-4)
hAP-0137	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-16 (HCC-4)
hAP-0140	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-23 (MPIF-1)
hAP-0148	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-23 (MPIF-1)
hAP-0148A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-26 (Eotaxin-3)
hAP-0151	Angio Proteomie	100ug	EUR 250

Anti-Mouse CCL-1 Antibody
103-M320	ReliaTech	100 µg	EUR 399
Description: Mouse CCL1, also known as TCA3, is a member of the CC beta family of chemokines. The human chemokine I309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA3. Mouse TCA3 and human I309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines. CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential Nlinked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP1α, MIP1β and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.

Anti-Mouse CCL-2 Antibody
103-M325	ReliaTech	100 µg	EUR 399
Description: Mouse CCL2 is a member of the β (CC) subfamily of chemokines. The mouse CCL2 gene was initially identified as a plateletderived growth factorinducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP1. Compared to human MCP1, mouse CCL2 has a 49 aa residue extension at the carboxyterminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C terminal extension could be due either to purification artifact or to posttranslational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP1 has limited activity on mouse cells.

Anti-Mouse CCL-3 Antibody
103-M333	ReliaTech	100 µg	EUR 399
Description: The macrophage inflammatory proteins 1α (or CCL3) and 1β, two closely related but distinct proteins, were originally co purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1α shares approximately 77% and 70% amino acid identity with human MIP1α and mouse MIP1β, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins are members of the β (CC) subfamily of chemokines. Both MIP1α and MIP1β are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1α and MIP1β preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1α has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1α has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP1β was reported to be much less active. The functional receptor for MIP1α has been identified as CCR1 and CCR5.

Anti-Mouse CCL-4 Antibody
103-M334	ReliaTech	100 µg	EUR 399
Description: CCL4, also known as macrophage inflammatory protein 1 beta (MIP1β), is a 12 kDa β chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP1α. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of Mtropic HIV1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV1 infection of T cells by inducing the downregulation of CCR5. Mature mouse CCL4 shares 77% and 86% amino acid sequence identity with human and rat CCL4, respectively.

Anti-Mouse CCL-5 Antibody
103-M335	ReliaTech	100 µg	EUR 399
Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa βchemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes. Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% amino acid (aa) sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M tropic) but not X4 (T tropic) strains of HIV. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M tropic HIV 1 infection of monocytes. Oligomerization of CCL5 on glycosaminoglycans is required for CCR1mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature.

Anti-Mouse CCL-8 Antibody
103-M337	ReliaTech	100 µg	EUR 399
Description: CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Anti-Mouse CCL-9 Antibody
103-M338	ReliaTech	100 µg	EUR 399
Description: Mouse CCL9/10 (also named MIP1γ and MRP2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the β (or CC) intercrine family of chemokines. Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteinecontaining CC subfamily of chemokines. This subfamily contains four Nterminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no humanto rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded Nterminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines. Mouse CCL9/10 is 75% aa identical to rat CCL9/10. Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at fulllength, but are converted to highly active forms upon Nterminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the Nterminus, generating a highly active, 8 kDa, 71 73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP1α and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a fulllength molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants.

Mouse Monoclonal anti-human CCL-2 (MCP-1)
hAP-0144	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-7 (MCP-3)
hAP-0156	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-8 (MCP-2)
hAP-0157	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-19 (MIP-3 beta)
hAP-0143	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-15 (MIP-1 delta)
hAP-0139	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-15 (MIP-1 delta)
hAP-0139A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-20 (MIP-3 alpha)
hAP-0145	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-4 (MIP-1 beta)
hAP-1166	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-3 (MIP-1 alpha)
hAP-0154	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-3 (MIP-1 alpha)
hAP-0154A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-3 (MIP-1 alpha)
hAP-0154B	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-28
mAP-0179	Angio Proteomie	100ug	EUR 250

Mouse CCL-5 ELISA Kit
EMC0240	Abclonal	96Tests	EUR 625.2

Rat Monoclonal anti-mouse CCL-3
mAP-0180	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-4
mAP-0181	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-5
mAP-0182	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-8
mAP-0184	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-9
mAP-0185	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-22 (MDC)
mAP-0175	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-17 (TARC)
mAP-0170	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-25 (TECK)
mAP-0177	Angio Proteomie	100ug	EUR 250

Anti-Human CCL-11 Antibody
101-M161	ReliaTech	100 µg	EUR 399
Description: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the aminoterminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

Anti-Human CCL-14 Antibody
101-M162	ReliaTech	100 µg	EUR 399
Description: CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20-93) is a 74 amino acid peptide that is also known as HCC1 (Hemofiltrate CC Chemokine1). It is a weak CCR1 agonist, however, an 8 amino acid Nterminal truncation (aa 28 93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC3, is a 90 amino acid peptide (aa 20-109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Anti-Human CCL-15 Antibody
101-M163	ReliaTech	100 µg	EUR 399
Description: CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF1, human HCC1, and mouse MIP1γ, respectively. The gene for MIP1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocytederived dendritic cells. Human MIP1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR1transfected cells.

Anti-Human CCL-28 Antibody
101-M185	ReliaTech	100 µg	EUR 399
Description: CCL-28 or MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Anti-Human CCL-14 Antibody
101-M246	ReliaTech	100 µg	EUR 399
Description: CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20-93) is a 74 amino acid peptide that is also known as HCC1 (Hemofiltrate CC Chemokine1). It is a weak CCR1 agonist, however, an 8 amino acid Nterminal truncation (aa 28 93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC3, is a 90 amino acid peptide (aa 20-109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Anti-Human CCL-11 Antibody
101-M256	ReliaTech	100 µg	EUR 399
Description: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the aminoterminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

Anti-Human CCL-13 Antibody
101-M257	ReliaTech	100 µg	EUR 399
Description: CCL13, also known as Monocyte Chemoattractant Protein4 (MCP4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR2 and CCR3.

Anti-Human CCL-15 Antibody
101-M258	ReliaTech	100 µg	EUR 399
Description: CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF1, human HCC1, and mouse MIP1γ, respectively. The gene for MIP1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocytederived dendritic cells. Human MIP1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR1transfected cells.

Anti-Human CCL-16 Antibody
101-M259	ReliaTech	100 µg	EUR 399
Description: CCL16, also known as HCC-4, is a CC chemokine that interacts with CCR1, CCR2, and CCR5. CCL16 is expressed weakly by NK cells, γδ T cells, and some T cells and is upregulated in monocytes by IL-10. CCL16 functions as a chemoattractant for monocytes and promotes the maturation of monocyte derived dendritic cells.

Anti-Human CCL-17 Antibody
101-M260	ReliaTech	100 µg	EUR 399
Description: CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1α, MIP1β, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

Anti-Human CCL-18 Antibody
101-M261	ReliaTech	100 µg	EUR 399
Description: CCL18, also known as PARC (Pulmonary and Activation-regulated Chemokine), is expressed by monocyte-derived dendritic cells and Th2 cytokine-stimulated macrophages. It functions as a chemoattractant for naïve T cells but not monocytes or neutrophils.

Anti-Human CCL-19 Antibody
101-M262	ReliaTech	100 µg	EUR 399
Description: CCL19, also known as MIP3β and ELC (EBI1Ligand Chemokine), is a 77 amino acid (aa) β chemokine that is distantly related to other β chemokines (20-30% aa sequence identity). The gene for MIP3β has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human β chemokines are clustered. MIP3β is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP3β is downregulated by the antiinflammatory cytokine IL10. Recombinant MIP3β is chemotactic for cultured human lymphocytes. MIP3β is a ligand for CCR7 (previously referred to as the EpsteinBarr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly upregulated in B cells infected with EpsteinBarr virus and T cells infected with herpesvirus 6 or 7.

Anti-Human CCL-20 Antibody
101-M264	ReliaTech	100 µg	EUR 399
Description: CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel β chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other β chemokines (20-28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR6 (previously referred to as GPRCY4, CKRL3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

Anti-Human CCL-21 Antibody
101-M265	ReliaTech	100 µg	EUR 399
Description: 6Ckine or CCL-21 is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (Secondary Lymphoid-tissue Chemokine), CCL21 and Exodus2, shows 21 33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxy-lterminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the gene for MIP3β/ ELC, another novel CC chemokine.

Anti-Human CCL-22 Antibody
101-M266	ReliaTech	100 µg	EUR 399
Description: CCL22, also named stimulated T cell chemotactic protein (STCP1) and MDC, is a CC chemokine initially isolated from clones of monocytederived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocytederived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV1 isolates and the T cell lineadapted isolate HIV1 IIIB, has been identified as CCL22. Based on aminoterminal sequence analysis, the major CD8 + T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

Anti-Human CCL-23 Antibody
101-M267	ReliaTech	100 µg	EUR 399
Description: CCL23, also known as Myeloid Progenitor Inhibitory Factor1 (MPIF1), is a CC chemokine that is produced by some myeloid cell lines and monocytederived dendritic cells. It binds CCR1 and inhibits the differentiation of progenitor cells that give rise to granulocytes and macrophages. CCL23 is also chemotactic for resting T cells, neutrophils, monocytes and dendritic cells.

Anti-Human CCL-24 Antibody
101-M268	ReliaTech	100 µg	EUR 399
Description: Eotaxin2, also named MPIF2 and Ckβ6, is a CC chemokine that is designated CCL24. Eotaxin2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C-terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin 2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP3, CCL3/MIP1α, and CCL11/Eotaxin, respectively. Eotaxin2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin2, Eotaxin and CCL13/MCP4 are mediated by the receptor CCR3.

Anti-Human CCL-25 Antibody
101-M269	ReliaTech	100 µg	EUR 399
Description: CCL25 (thymus-expressed chemokine) is a CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 aa precursor protein with a 23 aa signal peptide that is cleaved to yield a 127 aa mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 are chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 is also a specific agonist for the CCR9 chemokine receptor.

Anti-Human CCL-26 Antibody
101-M270	ReliaTech	100 µg	EUR 399
Description: Eotaxin-3 is a CC chemokine that signals through the CCR3 receptor. It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Recombinant human Eotaxin-3 is an 8.4 kDa protein containing 71 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Anti-Human CCL-27 Antibody
101-M271	ReliaTech	100 µg	EUR 399
Description: CTACK or CCL-27 is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant human CTACK is a 10.2 kDa protein containing 88 amino acid residues.

Anti-Human CCL-28 Antibody
101-M272	ReliaTech	100 µg	EUR 399
Description: CCL-28 or MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Rat Monoclonal anti-mouse CCL-27 (CTACK)
mAP-0178	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-21 (6Ckine)
mAP-0174	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-11 (Eotaxin)
mAP-0168	Angio Proteomie	100ug	EUR 250

Eotaxin / CCL-11 (Mouse) - ELISA Kit
EK-033-40	PHOENIX PEPTIDE	96 wells	EUR 603.72

Anti-Human CCL-1 Antibody
101-M255	ReliaTech	100 µg	EUR 399
Description: ccbe1 is not expressed in endothelial cells of lymph vessels, and it may be a component of the extracellular matrix. In zebrafish, ccbe1 expression was observed along the earliest migra­tion routes of endothelial cells that sprout from the posterior cardinal vein and migrate circuitously before developing into lymphatic vessels. ccbe1 might therefore be involved in guidance of these migrating cells.

Anti-Human CCL-2 Antibody
101-M263	ReliaTech	100 µg	EUR 399
Description: CCL2 is a βchemokine that is also known as monocyte chemotactic protein 1 (MCP1). It is produced by a variety of cell types and is chemotactic for monocytes.

Anti-Human CCL-3 Antibody
101-M273	ReliaTech	100 µg	EUR 399
Description: The macrophage inflammatory proteins 1α and 1β were originally copurified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1α refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP 1α. Mature MIP1α and MIP1β in both human and mouse share approximately 70% homology at the amino acid level. The MIP1 proteins are members of the β (CC) subfamily of chemokines. Both MIP1α and MIP1β are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1α and MIP1β preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1α has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1α has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP1α has been identified as CCR1 and CCR5.

Anti-Human CCL-5 Antibody
101-M274	ReliaTech	100 µg	EUR 399
Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the β (CC) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.

Anti-Human CCL-7 Antibody
101-M275	ReliaTech	100 µg	EUR 399
Description: MCP2 and CCL7 are two monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and CCL7 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. CCL7 also shares 58% amino acid identity with MCP2. CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites. Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Anti-Human CCL-8 Antibody
101-M276	ReliaTech	100 µg	EUR 399
Description: CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Rat Monoclonal anti-mouse CCL-12 (MCP-5)
mAP-0169	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-24 (Eotaxin-2
mAP-0176	Angio Proteomie	100ug	EUR 250

Anti-Human CCL-17 (TARC) Antibody
101-M164	ReliaTech	100 µg	EUR 399
Description: CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1α, MIP1β, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

Anti-Human CCL-27 (CTACK) Antibody
101-M184	ReliaTech	100 µg	EUR 399
Description: CTACK or CCL-27 is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant human CTACK is a 10.2 kDa protein containing 88 amino acid residues.

Rat Monoclonal anti-mouse CCL-1 (TCA-3)
mAP-0167	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-2 (MCP-1)
mAP-0172	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-28
hAP-5717	Angio Proteomie	50ug	EUR 400

Anti-Human CCL-5 (RANTES) Antibody
101-M191	ReliaTech	100 µg	EUR 399
Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the β (CC) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.

Mouse RANTES (CCL 5) Recombinant Protein
M10-038	ReliaTech	20 µg	EUR 196.35
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant murine RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Mouse RANTES (CCL 5) Recombinant Protein
M10-038S	ReliaTech	5 µg	EUR 92.4
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant murine RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola)

Background: Genome sequencing of all recognized eukaryotes on Earth guarantees unprecedented advances in organic sciences and in biodiversity-related utilized fields resembling environmental administration and pure product analysis. Advances in long-read DNA sequencing make it possible to generate high-quality genomes for a lot of non-genetic mannequin species. Nevertheless, long-read sequencing right this moment depends on sizable portions of high-quality, excessive molecular weight DNA, which is usually obtained from recent tissues. It is a problem for biodiversity genomics of most metazoan species, that are tiny and must be preserved instantly after assortment. Right here we current de novo genomes of two species of submillimeter Collembola. For every, we ready the sequencing library from excessive molecular weight DNA extracted from a single specimen and utilizing a novel ultra-low enter protocol from Pacific Biosciences. This protocol requires a DNA enter of solely 5 ng, permitted by a whole-genome amplification step.

Outcomes: The two assembled genomes have N50 values >5.5 and eight.5 Mb, respectively, and each comprise ∼96% of BUSCO genes. Thus, they’re extremely contiguous and full. The genomes are supported by an integrative taxonomy strategy together with placement in a genome-based phylogeny of Collembola and designation of a neotype for 1 of the species. Greater heterozygosity values are recorded within the extra cell species. Each species are devoid of the biosynthetic pathway for β-lactam antibiotics recognized in a number of Collembola, confirming the tight correlation of antibiotic synthesis with the species lifestyle.

Conclusions: It’s now attainable to generate high-quality genomes from single specimens of minute, field-preserved metazoans, exceeding the minimal contig N50 (1 Mb) required by the Earth BioGenome Undertaking.

Dietary supplementation of acetate-conjugated tryptophan alters feed consumption, milk yield and composition, blood profile, physiological variables, and warmth shock protein gene expression in heat-stressed dairy cows

The aim of this examine was to analyze the consequences of dietary supplementation of rumen-protected tryptophan (RPT) at 4 ranges on milk yield, milk composition, blood profile, physiological variables, and warmth shock protein gene expression in dairy cows underneath circumstances of moderate-severe warmth stress (MSHS, THI = 80~89). Sixteen early-lactating dairy cows (physique weight = 719 ± 66.Four kg, days in milk = 74.3 ± 7.1, milk yield = 33.55 ± 3.74 kg, means ± SEM) had been randomly assigned in a factorial association to one of many 4 therapies: management group (n = 4, no RPT supplementation), 15 g/d RPT (n = 4), 30 g/d RPT (n = 4), or 60 g/d RPT group per cow (n = 4) supplemented to the TMR.

The next dry matter consumption (DMI) and milk yield had been discovered within the 30 g RPT group in contrast with the opposite teams, and the three.5% fat-corrected milk yield, energy-corrected milk yield, milk fats, protein, β-casein, mono-unsaturated fatty acid, and poly-unsaturated fatty acid contents, and serum glucose content material had been noticed within the 30 g RPT group (p < 0.05). The milk lactose focus was considerably larger within the 30 g RPT group in contrast with the management and 60 g RPT teams (p < 0.05).

The plasma cortisol degree was decrease, whereas the serotonin and melatonin concentrations had been larger within the 30 g group in contrast with the opposite teams (p < 0.05). Warmth shock protein (HSP) 70 expression was downregulated within the management and 15 g RPT teams, whereas the expression of HSP90 and HSPB1 remained unchanged among the many teams. Specifically, the 30 g RPT group was thought-about to have an improved DMI, milk yield, and lactose focus, in addition to anti-heat stress results because of the simulation of serotonin and melatonin throughout MSHS.

Transthyretin amyloid fibrils alter major fibroblast construction, perform and inflammatory gene expression

Age-related wild kind transthyretin amyloidosis (wtATTR) is characterised by systemic deposition of amyloidogenic fibrils of misfolded transthyretin (TTR) within the connective tissue of many organs. Within the coronary heart this results in cardiac dysfunction, which is a big reason behind age-related coronary heart failure. The speculation examined is that TTR impacts cardiac fibroblasts in ways in which might contribute to fibrosis. When major cardiac fibroblasts had been cultured on TTR-deposited substrates, the F-actin cytoskeleton disorganized, focal adhesion formation decreased, and nuclear form was flattened.

Fibroblasts had sooner collective and single cell migration velocities on TTR-deposited substrates. Moreover, fibroblasts cultured on microposts with TTR deposition had lowered attachment and elevated proliferation above untreated. Transcriptomic and proteomic analyses of fibroblasts grown on glass lined with TTR confirmed vital upregulation of inflammatory genes after 48 hours, indicative of development in TTR-based ailments. Collectively, outcomes recommend that TTR deposited in tissue extracellular matrix might have an effect on each the construction, perform and gene expression of cardiac fibroblasts. As therapies for wtATTR are cost-prohibitive and solely sluggish illness development, higher understanding of mobile maladaptation might elucidate novel therapeutic targets.